ERG Dual Mouseholder
An electroretinography (ERG) procedure using mice and stimulus from an Espion ColorDome required the development of a mouse holder. The research group was getting too much noise to clearly see the electrical response of the data that they were collecting. Oxygen delivery and head stabilization were incorporated into the design to minimize the collected noise. The holders were made for two subjects to increase throughput of the experiment.
- Do not obstruct the eye of the animal.
- Direct oxygen towards both mice simultaneously.
- Provide openings for mouth and eye electrodes.
- Seamlessly incorporate the mouse holder onto the current testing setup on the side of a table with a heating bed.
- Restrict head movement of two mice facing each other while limiting the distance between them.
- Deliver anesthesia to the animal during imaging.
The stabilization of the mouse was done in two parts. The first part of the stabilization was done by an excavated area in the holder that was designed to fit the strain of mouse used for the ERG tests. This was created by doing a Boolean subtraction of a mouse mesh from the solid. The mouse mesh was created by 3d scanning a cadaver of a nude mouse. This provided the base mesh which was then cleaned up using the mesh tools that Rhino provides. The mold was scaled up to allow for slight tolerances in mouse sizes within the strain. The second part were small Velcro straps (not shown in images) that when from the flat half semi-circle to the other side of the holder.