Bone Marrow Imaging Stage
In vivo optical microscopy of cells in bone marrow in mice skull required a more functional imaging stage. Angular head shapes of mice made it difficult for scientists to get a high quality image due to varying depth of field from a non-flat surface. Movement of the mice during imaging due to minimal head stabilization also led to breathing artifacts during optical sectioning across the z-axis. I designed an imaging bed equipped with a fine adjustment pivoting system along with the necessary requirements for in vivo microscopy in the lab including temperature control, stabilization, anesthesia delivery, and vacuum of excess anesthesia.
- Create a bed that can adjust its angle to match the imaging area.
- Provide fine adjustments in tilting mechanism.
- Deliver anesthesia to the animal during imaging.
- Keep animal at body temperature of 37°C to prevent hypothermic shock in imaging lab
- Fit the holder under the limited space between the objective and the imaging table while keeping the image in focus.
- Restrict head movement of the imaged animal.
The front part of pivoting mechanism acts as a tuning system where the user can determine the amount of force necessary to pivot. This is done by tightening or loosening the nut on the nylon nut, which increases or decreases the compressive force on the holder. A brass rod was machined and held in place by a spacer, and a gear with a pin. The gear design was created in Rhino to match the commercial off the shelf (COTS) gear at a 10:1 gear ratio for precise angles. An additional knob was fabricated to turn the brass rod.