Wound Stabilization Bar
A research group at Novartis required an imaging stage that could clearly image mice skin wounds throughout the healing process. Breathing artifacts interfered with any time based imaging. Saline immersion with the previous bed was lacking, as the water would often leak off of the wound area over time. Imaging had to be stopped to reapply the saline solution. An existing bed was already in place, so I designed an attachment bar that was compatible with the existing bed to provide the image stabilization necessary for imaging while keeping saline water immersion of the wound area. All of the designs follow a similar principle. The skin on a mouse's back is very loose. By pulling the skin away from the body, breathing artifacts are eliminated. For this a two part system is necessary: an attachment to the wound area and a bar to pull this attachment up.
- Reduce the vibrations from breathing on the imaging area.
- Design a system where saline water stays on the wound.
- Adapt the design to the current mouse bed to eliminate the need of creating another bed.
- Allow for the device to be used for extended studies, with easy removal and attachment.
- Design the device to be lightweight and without any sharp edges for mouse comfort.
- Provide unobstructed access to the imaging area.
Often times in design, the first idea that you have will not work perfectly. Designing is an iterative process which requires an open mind to changes. My first design was to have a circular array of neodymium magnets pull a shim washer (attached to the mouse) upwards. This would have led to a very easy snap on attachment and removal system. Unfortunately, the shim washers rusted in the saline solution. To combat this, I tried changing the material of the shim washer, but with higher water resistance comes with less magnetism. I also tried coating the surface of the shim washer, but rust still occurred under thin acrylic coats as shown by the picture to the right. Larger coats of acrylic increased the imaging distance a couple of mm, rendering the imaging region out of focus for depth imaging.